Research in the Department of Orthopaedic Surgery is summarized as Biomaterials, Biomechanical, and Histology (please see links on the left).  

Additionally, Dr. Yufeng Dong, MD & PH.D. is involved in cellular research related to skeletal tissue regeneration, molecular mechanisms involving osteoarthritis, and developing translational solutions to develop cell based cartilage and repair of bone defects and fracture repairs.  Dr. Dong's Bio and information can be viewed below.

Yufeng Dong, MD & PhD

Yufeng Dong, MD & PhD

Contact Information:

Email Dr. Dong
Office:  BRI F3-26
Phone:  318.675.3252
Fax:  318.675.6186

Lab:  BRI F3-12
Phone:  318.675.3252

Bio:

Dr. Yufeng Dong, MD & Ph.D is the principle investigator in the Department of Orthopaedic Surgery. Dr. Dong received his Ph.D degree from Shanghai Jiaotong University and finished his postdoctoral training in University of Rochester. He has nearly 30 publication and received a significant funding from the NIH, Orthopaedic Research and Education foundation, Airlift Research Foundation and Lonza research Foundation to investigate the critical effects of stem cells on cartilage and bone regeneration.

Research Interests:
1. The molecular and cellular events underlying stem cell differentiation that leads to skeletal tissue regeneration, including Notch, Wnt and TGF beta signaling
2. The molecular mechanism underlying human osteoarthritis.
3. An emphasis on challenging clinical problems and translational solutions, including stem cell based cartilage, bone defect and fracture repair.

Specialized Techniques/Procedures:
1. Human and mouse bone marrow stem cell isolation and culture
2. Osteoblast and chondrocyte culture and differentiation
3. Mouse femur bone defect and tibia fracture surgery
4. Induced stem cell osteogenic and chondrogenic differentiation assay
5. Mouse limb bud stem cell isolation and micromass culture
6. Real time PCR for gene expression in tissue and cells
7. Flow Cytometry for cell sorting and surface marker assay
8. Bone and cartilage tissue histological analysis
9. Cell sheet culture and transplantation
10. Cells and biomaterials ectopic bone formation in vivo assay

Lab Members:

Current:
Sheila Rogers, Scientific Research Technologist
Dollie Smith, Research Associate
Guangxi Wang, Postdoctoral fellow
Emily Barsley, Medical student
Rachel Lee, Medical student

Past:
Bo Tian, Postdoctoral fellow
Junkui Sun, Postdoctoral fellow 
Xiaoya Xu, Postdoctoral fellow
Jordan Hanberry, Medical student
Clay Rodriguez, Medical student

Publications:
1. Tian Y, Xu Y, Xue T, Chen L, Shi B, Shu B, Xie C, Morandi M, Jaeblon T, Marymont J, Dong Y. Notch activation enhances mesenchymal stem cell sheet osteogenic potential by inhibition of cellular senescence. Cell death & disease 2017; 8: e2595. Online 2 February 2017.
2. Xifu Shang, Jinwu Wang, Zhengliang Luo, Yongjun Wang, Massimo M Morandi, John V Marymont, Matthew J Hilton, Yufeng Dong. Notch signaling indirectly promotes chondrocyte hypertrophy via regulation of BMP signaling and cell cycle arrest. Sci. Rep. 2016. 6, 25594; doi: 10.1038/srep25594.
3. Bo Tian, Wei Chen, Yufeng Dong, John V. Marymont, Yong Lei, Qinfei Ke, Yaping Guo and Zhenan Zhu. Silver nanoparticle-loaded hydroxyapatite coating: structure, antibacterial properties, and capacity for osteogenic induction in vitro. RSC Adv. 2016, 6(11): 8549-8562. DOI: 10.1039/C5RA25391H
4. Shang X, Luo Z, Wang X, Jaeblon T, Marymont JV, Dong Y. Deletion of RBPJK in Mesenchymal Stem Cells Enhances Osteogenic Activity by Up-Regulation of BMP Signaling. PLoS One. 2015 Aug 18; 10(8): e0135971. PMID: 26285013
5. Tian Y, Xu Y, Fu Q, Chang M, Wang Y, Shang X, Wan C, Marymont JV, Dong Y. Notch inhibits chondrogenic differentiation of mesenchymal progenitor cells by targeting Twist1. Mol Cell Endocrinol. 2015 Jan 14. PMID: 25596548
6. Dong Y, Long T, Wang C, Mirando AJ, Chen J, O'Keefe RJ, Hilton MJ. NOTCH-Mediated Maintenance and Expansion of Human Bone Marrow Stromal/Stem Cells: A Technology Designed for Orthopedic Regenerative Medicine. Stem Cells Transl Med. 2014 Nov 3. PMID: 25368376
7. Wang L, Jin F, Qin A, Hao Y, Dong Y, Ge S, Dai K. Targeting Notch1 signaling pathway positively affects the sensitivity of osteosarcoma to cisplatin by regulating the expression and/or activity of Caspase family. Mol Cancer. 2014 Jun 3;13:139. PMID: 24894297
8. Mirando AJ, Dong Y, Kim J, Hilton M. Isolation and culture of murine primary chondrocytes. Chapter 20, Book: Skeletal Development and Repair: Methods and Protocols, Methods in Molecular Biology. 2014; 1130:267-77. PMID: 24482180
9. Long T, Zhu Z, Awad H, Schwarz E, Hilton M, Dong Y. Mesenchymal stem cell sheets enhance structural allograft healing of critical-sized femoral defects in mice. Biomaterials. 2014 Mar; 35(9):2752-9. PMID: 24393269
10. Gao L, Sheu TJ, Dong Y, Hoak DM, Zuscik MJ, Schwarz EM, Hilton MJ, O'Keefe RJ, Jonason JH. TAK1 regulates SOX9 expression in chondrocytes and is essential for postnatal development of the growth plate and articular cartilages. J Cell Sci. 2013 Oct 21. PMID: 24144697
11. Yao Z, Li Y, Yin X, Dong Y, Xing L, Boyce BF. NF-κB RelB negatively regulates osteoblast differentiation and bone formation. J Bone Miner Res. 2013 Oct 1. doi: 10.1002/jbmr.2108.
12. Wang J, Wang X, Holz J, Rutkowski T, Wang Y, Zhu Z, Dong Y. Runx1 is Critical for PTH-induced Onset of Mesenchymal Progenitor Cell Chondrogenic Differentiation. PLOS ONE. 2013 Sep 18;8(9):e74255. doi: 10.1371/journal.pone.0074255.
13. Kohn A, Dong Y, Mirando AJ, Jesse A, Honjo T, Zuscik M, O’Keefe R, Hilton M. Cartilage-specific RBPjκ-dependent and -independent Notch signals regulate cartilage and bone development. Development. 2012 Mar; 139(6):1198-212. PMC3283126.
14. Tian Y, Xu Y, Fu Q, Dong Y. Osterix is required for Sonic Hedgehog-Induced Osteoblastic MC3T3-E1 Cell Differentiation. Cell Biochem Biophys. 2012 Dec; 64(3): 169-76. PMID: 22648388.
15. Dong Y, Jesse A, Kohn A, Gunnell L, Honjo T, Zuscik M, O’Keefe R, Hilton M. RBPJk-dependent Notch signaling maintains and expends mesenchymal stem/progenitor cells during skeletal development, Development, 2010 May;137(9):1461-71
16. Eliseev R, Dong Y, Sampson E, Zuscik M, Schwarz EM, O'Keefe RJ, Rosier R, Drissi H. Runx2-mediated activation of the Bax gene increase osteosarcoma cell sensitivity to apoptosis. Oncogene. 2008 Jun 5;27(25):3605-14
17. Dong Y, Soung DY, Chang Y, Enomoto M, Paris M, Schwarz EM, O'Keefe RJ, Drissi H. TGF-b and Wnt signaling stage-specifically regulate chondrocyte differentiation through Twist1. Molecular Endocrinology. 2007 Nov; 21(11):2805-20
18. Soung DY, Dong Y, Wang Y, Schwarz EM, O'Keefe RJ, Drissi H. Runx3/AML2/Cbfa3 Regulates Early and Late Chondrocyte Differentiation. J Bone Miner Res. 2007 Aug; 22(8): 1260-70.
19. Dong Y, Soung DY, Schwarz EM, O'Keefe RJ, Drissi H. Wnt induction of chondrocyte hypertrophy through the Runx2 transcription factor, J Cellular Physiology. 2006 Jul; 208(1):77-86
20. Wang YJ, Shi Q, Lu WW, Cheung KC, Darowish M, Li TF, Dong YF, Zhou CJ, Zhou Q, Hu ZJ, Liu M, Bian Q, Li CG, Luk KD, Leong JC. Cervical intervertebral disc degeneration induced by unbalanced dynamic and static forces: a novel in vivo rat model.
Spine. 2006 Jun 15; 31(14):1532-8.
21. Wang YJ, Shi Q, Sun P, Zhou Q, Darowish M, Li TF, Dong YF, Lu WW, Leong JC. Insulin-like growth factor-1 treatment prevents anti-Fas antibody-induced apoptosis in endplate chondrocytes. Spine. 2006 Apr 1; 31(7):736-41.
22. Nishio Y, Dong Y, Paris M, O'Keefe RJ, Schwarz EM, Drissi H. Runx2 mediated regulation of the zinc finger osterix/SP7 gene. Gene. 2006 May 10; 372:62-70.
23. Dong Y, Hicham Drissi, Mo Chen, Di Chen, Michael J Zuscik, Edward M. Schwarz; and Regis J. O’Keefe. Wnt mediated regulation of chondrocyte maturation: modulated by TGF-b. J Cellular Biochemistry. 2005 Aug 1; 95(5):1057-68
24. Wang Y, Ruth B, Dong Y, Schwarz EM, O'Keefe RJ, Drissi H. Runx1/AML1/cbfa2 mediate onset of mesenchymal cell differentiation towards chondrogenesis. J Bone and Mineral Research, 2005 Sep; 20(9):1624-36
25. Dong Y, Smith N, Lian JB, Pratap J, Kingsley PD, van Wijnen AJ, Stein JL, Schwarz EM, O'Keefe RJ, Stein GS, Drissi MH. Overlapping expression of Runx1 (Cbfa2) and Runx2 (Cbfa1) transcription factors supports cooperative induction of skeletal development. J Cellular Physiology. 2005 Apr; 203 (1):133-43.
26. Li TF, Dong Y, Ionescu AM, Rosier RN, Zuscik MJ, Schwarz EM, O'Keefe RJ, Drissi H. Parathyroid hormone-related peptide (PTHrP) inhibits Runx2 expression through the PKA signaling pathway. Experimental Cell Research. 2004 Sep 10; 299(1):128-36.